The purpose of this research was the Identify the relationship between organizational justice and quality of work life and the mediating role of organizational culture from the point of view of employees at Shahid Beheshti University. For this purpose from survey - descriptive were used. To collect data, the two researcher-made questionnaires and the standardized questionnaire of 263 employees were used. For analyzing the data of research descriptive and inferential statistical methods (variate T-test, bivariate T-test, Pearson correlation, structural equation, ANOVA) were used. Research findings indicated that between organizational justice and those components with quality of working life, between organizational justice and those components with organizational culture, between organizational cultures with quality of working life is a significant positive correlation. Also results indicated that organizational culture well play the role of mediator on the relationship between organizational justice and the quality of working life, Regarding the variables studied, research findings indicated that organizational justice and the quality of working life was unfavorable situation and organizational culture was relatively desirable.